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KMID : 0380220070400060986
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Journal of Biochemistry and Molecular Biology
2007 Volume.40 No. 6 p.986 ~ p.1001
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Differentially Expressed Genes under Cold Acclimation in Physcomitrella patens
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Sun Ming-Ming
Li Lin-Hui
Xie Hua
Ma Rong-Cai
He Yi-Kun
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Abstract
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Cold acclimation improves freezing tolerance in plants. In higher plants, many advances have been made toward identifying the signaling and regulatory pathways that direct the low-temperature stress response; however, similar insights have not yet been gained for simple nonvascular plants, such as bryophytes. To elucidate the pathways that regulate cold acclimation in bryophytes, we used two PCR-based differential screening techniques, cDNA amplified fragment length polymorphism (cDNAAFLP) and suppression subtractive hybridization (SSH), to isolate 510 ESTs that are differentially expressed during cold acclimation in Physcomitrella patens. We used realtime RT-PCR to further analyze expression of 29 of these transcripts during cold acclimation. Our results show that cold acclimation in the bryophyte Physcomitrella patens is not only largely similar to higher plants but also displays distinct differences, suggests significant alteration during the evolution of land plants.
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KEYWORD
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cDNA-amplified fragment length polymorphism cDNA-AFLP), Cold acclimation, Gene expression profile, Physcomitrella patens (Hedw.), Real-time RT-PCR, Suppression subtractive hybridization (SSH)
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